As conclusion, pH, temperature and light have great impact to the colour degradation of betalain pigment in extracted red dragon fruit juice during storage. In the present study, light has been the major factor of colour deterioration. Exposure of light caused colour lost up to 50% after one week storage in room temperature. Pre-heat treatment of red dragon fruit juice before storage did not show significant effect in preventing colour degradation. In fact, high temperature (85°C) led to almost 30% of colour lost in the initial stage of storage. Sample subjected to heat treatment up to 50°C slightly reduced the juice colour. The treatment failed to preserve the colour of red dragon fruit for all storage in room temperature. Ascorbic acid as well, did not exhibit promising results in red dragon fruit juice stored in room temperature. The best condition in preserving betalain pigment in red dragon fruit juice is pH 5.0 at 4°C. Sunlight should be totally avoided to preserve the colour of the pigment. The next challenge of the red dragon fruit pigment is to incorporate it into food products. The stability of the pigment in the food products will lead us to the answer whether the pigment is an alternative to red beet betalain.